Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

Table 2 Number of cells in the inner cell mass (ICM), trophectoderm (TE), total cells, and ICM:TE ratio of hatched blastocysts in the control and laser groups in experiment 1

	Control	1-hole	2-hole	3-hole	P
	(n=44)	(n=19)	(n=14)	(n=12)	P
ICM	23.6±4.6	19.5±3.8	16.5±3.0	15.2±2.3	0.000^a
TE	68.7±15.9	59.7±14.1	53.1±8.4	50.3±9.8	0.000^b
ICM/TE	0.4±0.1	0.3±0.1	0.3±0.1	0.3±0.1	0.184
Total	92.2±18.3	79.2±16.4	68.9±9.4	65.5±10.1	0.000^c

Values are mean ± standard deviation (SD).
One-way ANOVA with Scheffe post-hoc test when P<0.0125 (P<0.05/4).
^a control vs. 1-hole group (P=0.004), control vs. 2-hole group (P=0.000), control vs. 3-hole group (P=0.000), and 1-hole vs. 3-hole group (P=0.039).
^b control vs. 2-hole group (P=0.006), and control vs. 3-hole group (P=0.002).
^c control vs. 1-hole group (P=0.036), control vs. 2-hole group (P=0.000), and control vs. 3-hole group (P=0.000).

ISSN: 1477-7827