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Table 1 Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes

From: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

   Primer sequence
Forward Reverse
qAMP H19 5′-AGCCGTTGTGAGTGGAAAGA-3′ 5′-CATAGCGGCTTCGGACATT-3′
Snrpn 5′-CTCCTCAGAACCAAGCGTCT-3′ 5′-ATTCCGGTCAGAGGGACAGA-3′
Peg3 5′-GGTGTCCCGCAGCCCTTG-3′ 5′-CGGAGCACAGCACTCTACGC-3′
chr9:106724005-106724149 5′-GATCTATTCCTTCCTTTACTTT-3′ 5′-TCCTGGGAAATGAAGTTT-3′
Peg3 qRT-PCR 5′-TTGGACTGGACAGAGATGATGACA-3′ 5′-ATTCTGGTATGACTCGGCATCCT-3′
Snrpn 5′-AGGCCCATCCCAGCAGGTCAT-3′ 5′-GCGGGTACTGGGTTGGGGCTC-3′
H19 5′- CTTGTCGTAGAAGCCGTCTGTTC-3′ 5′- GTAGCACCATTTCTTTCATCTTGAGG-3′
Ppia   5′- CGCGTCTCCTTCGAGCTGTTTG-3′ 5′- TGTAAAGTCACCACCCTGGCACAT-3′