Table 1 Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes

qAMP

H19

5′-AGCCGTTGTGAGTGGAAAGA-3′

5′-CATAGCGGCTTCGGACATT-3′

Snrpn

5′-CTCCTCAGAACCAAGCGTCT-3′

5′-ATTCCGGTCAGAGGGACAGA-3′

Peg3

5′-GGTGTCCCGCAGCCCTTG-3′

5′-CGGAGCACAGCACTCTACGC-3′

chr9:106724005-106724149

5′-GATCTATTCCTTCCTTTACTTT-3′

5′-TCCTGGGAAATGAAGTTT-3′

Peg3

qRT-PCR

5′-TTGGACTGGACAGAGATGATGACA-3′

5′-ATTCTGGTATGACTCGGCATCCT-3′

Snrpn

5′-AGGCCCATCCCAGCAGGTCAT-3′

5′-GCGGGTACTGGGTTGGGGCTC-3′

H19

5′- CTTGTCGTAGAAGCCGTCTGTTC-3′

5′- GTAGCACCATTTCTTTCATCTTGAGG-3′

Ppia

5′- CGCGTCTCCTTCGAGCTGTTTG-3′

5′- TGTAAAGTCACCACCCTGGCACAT-3′