DNA methylation and expression levels of Peg3 in placentas at mid-gestation. (A, B) Non-diabetic pronuclear embryos were transferred to normal/diabetic (NN/ND) pseudopregnant female and the DNA methylation and expression levels of Peg3 (n, NN:ND = 12:15) in 10.5d placentas were analyzed by qAMP and qRT-PCR, respectively. (A) methylation level of Peg3 in dpc10.5 placenta; (B) expression of Peg3 in placenta. (C, D) DN’s (n = 15 from 4 litters) and NN’s (n = 15 from 4 litters) placentas were collected at mid-gestation. DNA was digested with HpaIIor McrBC and amplified using real-time PCR. (C) the methylation in DMRs of Peg3 in placentas; (D) Relative expression levels of Peg3 in placentas. Data were presented as mean ± SD. *P < 0.05, **P < 0.01.