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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Profiles of cytokines secreted by isolated human endometrial cells under the influence of chorionic gonadotropin during the window of embryo implantation

Figure 2

Representative Western blot analysis of selected cytokines. Immunopositive images of CCL2, GMCSF, IL-6, LIF, PDGFBB and VEGF in conditioned media with isolated human endometrial epithelial cells (Group 1), stromal cells (Group 2) and mixed cells (Group 3) treated without or with rhCG (100 IU/ml) are shown. Conditioned media from different groups (20 μg of protein determined by Bradford assay) were subjected to electrophoretic separation followed by immunoblot analysis. The relative optical densities were measured by integrated image analysis per μg of protein.

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