Figure 1

Immunocytochemical characterisation of isolated endometrial cell populations in primary culture. Isolated human mid-luteal phase endometrial epithelial cells (A, D, G, J, M, P), stromal cells (B, E, H, K, N, Q) and mixed cells (C, F, I, L, O, R) were allowed to attach to the collagen biomatrix and subjected to immunostaining for cytokeratin (green; A-C), vimentin (red; D-F), CD45 (green; G-I), vW factor (red; J-L), and hCG binding (green; M-O) (detected with biotinylated rhCG) was determined in each cell type and in non-immune sera from mouse and goat (P) and non-immune sera from mouse and rabbit (Q) and biotinylated rhCG alone (R) followed by immunofluorescence detection was performed as described in the Methods section. Nuclei were counterstained with DAPI (blue). Bars = 50 μm.