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Table 2 Average values for sample collection parameters in experiment 1 and 2

From: Follicular expression of follicle stimulating hormone receptor variants in the ewe

Group Time to euthanasia Mean cycle length Number of follicles Luteal tissue (#) Serum progesterone
Exp 1 (hours) (days) ≤ 2 mm 2.1-4 mm 4.1-6 mm ≥ 6.1 mm CH CL CA (ng/ml)
24 22.83 ± 0.24 16.31 ± 0.48 11 ± 8.3 5.7 ± 0.7 0 2 ± 0 0 0.3 ± 0.3 2.0 ± 0.6 0.43 ± 0.06
36 36.64 ± 0.83 16.80 ± 0.28 15.7 ± 5.0 8.3 ± 2.7 0.3 ± 0.3 0.3 ± 0.3 1.6 ± 0.3 1.0 ± 0.6 2.0 ± 0.6 0.54 ± 0.14
48 49.33 ± 2.16 13.10 ± 4.85 14.5 ± 2.5 6.0 ± 3.0 0.5 ± 0.5 0 2.5 ± 0.5 1.5 ± 1.5 1.5 ± 0.5 0.55 ± 0.13
  1. Ewes were monitored through three estrous cycles then euthanized 24 (n = 3), 36 (n = 3), or 48 (n = 2) hours after the onset of the next estrus. Data are presented as means ± SEM. Ovarian structures were measured on the external surface of the ovary with a transparent ruler (to the nearest 0.5 mm) and recorded. All visible follicles were aspirated and follicular fluid from each pair of ovaries was pooled according to follicular diameter: small (≤ 2.0 mm), medium (2.1-4.0 mm), large (4.1-6.0 mm; gene expression in large follicles was not assessed due to low cell numbers) and preovulatory (≥ 6.1 mm). Blood was collected immediately prior to euthanasia.