Expression of gonadotropin receptors across different sized follicles. Cells were collected by centrifugation from follicular fluid aspirated from small (≤ 2.0 mm; white bars) or medium (2.1-4.0 mm; gray bars) follicles. Results from real time PCR are expressed as least squares means of expression relative to β-actin (2-∆Ct ×1000; arbitrary units) ± SEM. Bars within graphs with differing superscripts are different (p < 0.05). Panels A), B), and D) Relative expression of FSHR-1, FSHR-2, and LHR did not significantly differ in small versus medium follicles. Panel C) Relative expression of FSHR-3 was higher in medium than small follicles. Overall, FSHR-3 expression was the only transcript that differed between small and medium follicles.