Figure 1

Location of primers to specifically amplify FSH receptor splice variants. Primer pairs used for real time PCR were designed to result in amplicons unique to each specific form of the receptor as follows: FSHR-1—primer annealing sites within the unique region of exon 10 only found in this variant form of the receptor; FSHR-2—upstream primer annealing sites overlapping the exon 10 and 11 boundary only found in this variant; FSHR-3—upstream primer annealing sites overlapping the exon 8 and 11 boundary only found in this variant. Predicted sizes of amplicons were verified on an agarose gel (not shown), and melt curve analysis was performed to verify single products.