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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Analysis of 20alpha-hydroxysteroid dehydrogenase expression in the corpus luteum of the buffalo cow: effect of prostaglandin F2-alpha treatment on circulating 20alpha-hydroxyprogesterone levels

Figure 6

Activity of cytosolic 20α-HSD in luteal tissue of pseudo pregnant rats and buffalo cows. The enzyme activity is expressed as nmoles NADPH min-1 mg-1 with 1 unit of activity defined as the amount of enzyme that can induce formation of 1 nmole NADP min-1 mg-1 extract used at 37 oC. (A) Specific activity of 20α-HSD at 0 and 24 h post PGF treated pseudo pregnant rat CL cytosolic extract. (B) Specific activity of 20α-HSD at 0, 3, 6, 18, 36 and 60 h post PGF treated buffalo CL cytosolic extract. The cytosolic extract from the luteal tissue collected without PGF treatment (0 h) was designated as control. Each bar represents mean ± SEM, n = 5/time point for pseudo pregnant rats and n = 3/time point for buffalo cows, **p < 0.01, 0 h versus different time points.

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