Activity of cytosolic 20α-HSD in luteal tissue of pseudo pregnant rats and buffalo cows. The enzyme activity is expressed as nmoles NADPH min-1 mg-1 with 1 unit of activity defined as the amount of enzyme that can induce formation of 1 nmole NADP min-1 mg-1 extract used at 37 oC. (A) Specific activity of 20α-HSD at 0 and 24 h post PGF2α treated pseudo pregnant rat CL cytosolic extract. (B) Specific activity of 20α-HSD at 0, 3, 6, 18, 36 and 60 h post PGF2α treated buffalo CL cytosolic extract. The cytosolic extract from the luteal tissue collected without PGF2α treatment (0 h) was designated as control. Each bar represents mean ± SEM, n = 5/time point for pseudo pregnant rats and n = 3/time point for buffalo cows, **p < 0.01, 0 h versus different time points.