Chromatograms of 20α-OHP and P
in buffalo cow serum samples before and after PGF
treatment. (A) The standard mixture of steroids comprising of 5 ng each of 20α-OHP and P4 was used during HPLC run. The peak for 20α-OHP and P4 were noted down at 19.1 and 22.8 min, respectively. Chromatogram showing steroids extracted from serum collected at 0 h (B) and 18 h (C) post PGF2α treatment in the buffalo cow are shown.