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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Analysis of 20alpha-hydroxysteroid dehydrogenase expression in the corpus luteum of the buffalo cow: effect of prostaglandin F2-alpha treatment on circulating 20alpha-hydroxyprogesterone levels

Figure 5

Chromatograms of 20α-OHP and P 4 in buffalo cow serum samples before and after PGF treatment. (A) The standard mixture of steroids comprising of 5 ng each of 20α-OHP and P4 was used during HPLC run. The peak for 20α-OHP and P4 were noted down at 19.1 and 22.8 min, respectively. Chromatogram showing steroids extracted from serum collected at 0 h (B) and 18 h (C) post PGF treatment in the buffalo cow are shown.

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