Effect of PGF
treatment on serum progesterone, luteal 20α-HSD and Nur77 mRNA expressions. Buffalo cows received intramuscular injection of 500 μg of PGF2α on day 11 of estrous cycle and blood and luteal tissue samples were collected at 0, 3, 6 and 18 h post PGF2α treatment. (A) Mean (±SEM) circulating serum progesterone (P4) concentration immediately before (0 h) and after (3, 6 and 18 h) the PGF2α treatment in buffalo cows, (***p < 0.001). (B) qPCR expression of 20α-HSD mRNA levels in the CL from buffalo cows collected at 0, 3, 6 and 18 h post PGF2α treatment with the expression normalized with L19 mRNA. (C) qPCR expression of Nur77 mRNA levels in the CL from buffalo cows collected at 0, 3, 6 and 18 h post PGF2α treatment and the expression was normalized with L19 mRNA. The results are shown as fold changes of mRNA expression compared with that at 0 h PGF2α. Bar represents mean ± SEM, n = 3, ***p < 0.001 and **p < 0.01 versus control (0 h).