Expression of SMILE mRNA and protein in the mouse embryos during pre-implantation. (A) Immunofluorescence analyses of SMILE protein in embryos from the zygote to the blastocyst stage. The red colour represents SMILE staining, and the blue colour indicates nuclear staining. Bar = 20 μm. (B) Analysis of SMILE mRNA levels using real-time PCR in the mouse embryos. The values represent the mean ± S.E.M from three independent experiments. Bars with different letters are significantly different (P < 0.05).