Figure 1From: Age-associated telomere shortening in mouse oocytesScheme of MII oocyte collection and expression analysis of Tert transcripts and protein. (A) Diagram showing the study design for collecting fresh oocyte retrieved from young or reproductively-aged females, and oocytes aged in vivo or in vitro after ovulation (OPU: oocyte pick up). (B) Q-PCR analysis of Tert in MII oocytes. Gapdh was used as an internal standard for gene expression (n: number of replicated experiments). The error bars show mean ± SEM. *P < 0.05; ****P < 0.0001. (C) Immunocytochemical analysis of TERT expression (yellow). Nuclei were stained with DAPI (blue). Fresh oocytes from reproductively-aged females exhibited weaker TERT signal intensity than fresh oocytes from young females. (D) Quantification of relative TERT fluorescence intensities shown in 1C (N: total number of oocytes). The error bars show mean ± SEM. ****P < 0.0001.Back to article page