PreImplantation factor (PIF) detection in maternal circulation in early pregnancy correlates with live birth (bovine model)

Table 1 PIF detection by ELISA

Dilution factor (DF)	Observed (ng/ml)	Expected ng/ml	Recovery %
Dilution factor (DF)	X DF	(neat value)	Recovery %
High spike concentration (n = 3)
Neat	570	625	91.2
1;2	633		101.3
1;4	615		98.3
1;8	571		91.3
Medium spike concentration (n = 3)
Neat	154	156	98.7
1;2	143		91.5
1;4	139		88.9
1;8	149		95.2
Low spike concentration (n = 3)
Neat	71	78	91.0
1;2	69		88.9
1;4	74		95.2
1;8	68		87.2

ELISA is linear at different dilutions and spiked PIF recovery is >90%. Observed values were assessed relative to the assay standard curve produced by a PIF standard prepared in the steer sera.

ISSN: 1477-7827