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Table 1 PIF detection by ELISA

From: PreImplantation factor (PIF) detection in maternal circulation in early pregnancy correlates with live birth (bovine model)

Dilution factor (DF) Observed (ng/ml) Expected ng/ml Recovery %
X DF (neat value)
High spike concentration (n = 3)
Neat 570 625 91.2
1;2 633 101.3
1;4 615 98.3
1;8 571 91.3
Medium spike concentration (n = 3)
Neat 154 156 98.7
1;2 143 91.5
1;4 139 88.9
1;8 149 95.2
Low spike concentration (n = 3)
Neat 71 78 91.0
1;2 69 88.9
1;4 74 95.2
1;8 68 87.2
  1. ELISA is linear at different dilutions and spiked PIF recovery is >90%. Observed values were assessed relative to the assay standard curve produced by a PIF standard prepared in the steer sera.