Table 1 List of antibody targeting proteins of interest used for RPPA and WB
Antibody name | Company & reference | Species | Validated for RPPA | Dilution RPPA | Dilution WB |
|---|---|---|---|---|---|
ERK2 (C-14) | Santa Cruz Biotechnology - #sc-154 | Rabbit - pAb | Yes | 1:1000 | 1:10000 |
GAPDH (D16H11) | Cell Signaling - #5174 | Rabbit - mAb | No | 1:1000 | 1:10000 |
SRC (36D10) | Cell Signaling - #2109 | Rabbit - mAb | No | 1:500 | 1:1000 |
VCL | Sigma-Aldricht - #V9131 | Mouse - mAb | No | 1:1000 | 1:10000 |
RGS2 | Abnova - #H00005997-M01 | Mouse - mAb | No | 1:500 | 1:5000 |