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Table 4 Time and dose-dependent effects of norepinephrine on pregnenolone production

From: Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions

Time (h)

Norepinephrine (M)

0

10-9

10-8

10-7

0

2.1 ± 1.2a

2.1 ± 1.2a

2.1 ± 1.2a

2.1 ± 1.2a

48

2.5 ± 1.6a

4.7 ± 1.4a

4.6 ± 1.3a

4.4 ± 1.2a

96

9.0 ± 1.6b

12.4 ± 1.4b

10.6 ± 1.4b

11.9 ± 1.4b

  1. Time and dose-dependent effects of norepinephrine on pregnenolone (ng/ml) production by highly estrogenic bovine granulosa cells cultivated with 0.1% PVA supplementation.
  2. a, b Means within a column with unlike superscripts differ (P < 0.05); values are least square means ± SEM (n=5 independent cultures).