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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Estrous cycle-dependent changes of Fas expression in the bovine corpus luteum: influence of keratin 8/18 intermediate filaments and cytokines

Figure 5

Fluorescent detection of K8/K18 filaments and microtubules in control and acrylamide-treated cultures of bovine luteal cells. K8/K18 filaments (green fluorescence) and microtubules (red fluorescence) were immunostained in cultured bovine luteal cells following 4 h exposure to vehicle (Control, Figure 5A and B) or 5mM acrylamide (Figure 5C and D). Cells in control cultures exhibited a filamentous, K8/K18 intermediate filament network which spanned the cytoplasm (Figure 5A). Microtubles of these cells was similarly filamentous (red fluorescence; Figure 5B). Conversely, cells of acrylamide-treated cultures exhibited peri-nuclear aggregation of K8/K18 filaments (Figure 5C), yet the microtubules were unaffected (Figure 5D). Magnification: 40x.

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