SOD1 protein expression and total SOD activity in LECs. Effects of PGF and H2O2 on the expression of SOD1 protein (Figure3A, 3C) and total SOD activity (Figure3B, 3D) in cultured bovine LECs. The cells were cultured with 1 μM PGF and 10 μM H2O2 for 2 and 24 h. Photographs showed the expression of SOD1 protein (23 kDa) and ACTB (42 kDa). The amount of SOD1 protein is expressed relative to the amount of ACTB. Total SOD activity was determined by a colorimetric method using SOD Assay kit-WST. Asterisks indicate significant differences between untreated cells and treated cells (P<0.05). All values represent mean ± SEM of four separate experiments.