Figure 4

Targeting HLX1 reduces the cell invasion and the production of MMP2 in response to HGF. (A) HTR-8/SVneo cells were either not transfected (NT), or transfected with siCtrl or siHLX. The invasion of transfected cells across matrigel was determined by a transwell assay. Images of representative invasive cells are shown on the left, and quantifications are shown on the right. (B) HTR-8/SVneo cells were transfected with either siCtrl or siHLX1 and treated with or without 20 ng/mL HGF. The invasion of transfected cells across matrigel was determined by a transwell assay. Images of representative invasive cells are shown on the left, and quantifications are shown on the right. All quantitative data are presented as mean ± SD for three independent experiments. *P < 0.01, as compared to the other two groups. (C) HTR-8/SVneo cells were treated as indicated. The MMP2 and MMP9 levels in the conditioned medium from the cells were measured by gelatin zymography; conditioned medium collected from HT1080 cells were used as a positive control (PC).