Targeted silencing of HLX1 inhibits basal and HGF-induced cell growth. (A) HTR-8/SVneo cells were either not transfected (NT), or transfected with siCtrl or siHLX and cell growth at 0, 24, 48, 72 and 94 h after transfection was determined by MTT assay. (B) HTR-8/SVneo cells were transfected with either siCtrl or siHLX1 at time 0, and treated with or without 20 ng/mL HGF. Cell growth at 0, 24, 48, 72 and 94 h after transfection was determined by MTT assay with the absorbance of viable cells read at 490 nm (OD490). All quantitative data are presented as mean ± SD for three independent experiments. *P < 0.01, as compared to NT cells or siCtrl-transfected cells (in A), and to siCtrl + HGF or siHLX1 cells (in B). #P < 0.01, as compared to siHLX1 + HGF cells (in B).