HGF up-regulates the expression of HLX1 in HTR-8/SVneo cells. (A) HTR-8/SVneo cells were treated with the indicated concentrations of HGF for 48 h. The steady-state mRNA level of HLX1 was examined by qPCR and normalized to a β-actin internal control. The ratio of HLX1/β-actin mRNA in cells treated with 0 ng/mL HGF was arbitrarily defined as 1. Data are presented as mean ± SD for three independent experiments. *P < 0.01, vs. 0 ng/mL HGF. (B) HTR-8/SVneo cells were treated with 0 ng/mL (−) or 20 ng/mL (+) HGF for 48 h. The protein level of HLX1 was determined by Western immunoblot and normalized to β-actin internal control. A representative gel image is presented in the upper panel and quantification of HLX1 to β-actin signal ratio from three independent experiments is shown in the lower panel. The ratio in –HGF cells was arbitrarily defined as 1. *P < 0.01, vs. 0 ng/mL HGF.