Figure 4

Expression dynamics of seven miRNAs in sperm from six bulls with varying fertility. Mean Ct values from the High fertility animals were used as reference points and Ct values for groups (high and low fertility) were used to calculate the fold change from the reference points for high fertility group using reference point Ct values according to 2-ΔΔCt method as described by Livak and Shimitgen [25]. Data were presented as mean value ± SEM for each miRNA and * indicates statistical significance at p < 0.05.