Figure 1

Overview of gene expression profiles and results of protein detection. Overview of gene expression profiles (RT-PCR) and results of protein detection (ELISA) in cell culture supernatant of vehicle and corticotropin-releasing hormone (CRH) (1.0 and 2.0 μg/ml) treated trophoblasts at 48 and 72 h. Top row: Leptin gene expression (Leptin, blue bars), Leptin protein secretion (Leptin P, red bars). Middle row: Syncytin-1 (Syn1) (blue bars), β-hCG (red bars). Bottom row: CRH-R1 (blue bars), CRH-R2 (red bars), 11β-HSD2 (green bars). Displayed are values relative to the control value at the designated time-point as mean ± SEM, * = p < 0.05.