Figure 6

Testicular cross-sections of 127 day-old control (Figure6A) and doxorubicin-treated (Figure6B-D) rats submitted to transferrin labeling. The Figures 6A-B show intense transferrin labeling in the seminiferous epithelium (stage VII). However, in the Figure 6A (control group), the labeling is more abundant than in the Figure 6B (doxorubicin-treated group). In the control group (Figure 6A), transferrin labeling is observed in the Sertoli cell cytoplasm (thick arrow) and nucleus (inset, thin arrow) as well as in the elongated spermatids (arrowhead). In the doxorubicin-treated group (Figure 6B) transferrin labeling is observed in the Sertoli cell cytoplasm (thick arrow) and in the elongated spermatids (arrowhead), but not in the Sertoli cell nucleus (inset, thin arrow). The Figure 6C shows two seminiferous tubule cross-sections containing large portions of sloughed seminiferous epithelium (stars). In one of them, transferrin positive Sertoli cell cytoplasm is observed (arrow). In the Figure 6D, a tubular section with intense germ cell depletion shows weak Sertoli cell cytoplasm immunolabeling (thin arrows) and no labeling in the Sertoli cell nucleus (arrowheads). Intraepithelial vacuole (thick arrow). Note the intense labeling in the interstitial tissue (Figure 6A-D; asterisks).