Figure 5

Testicular cross-sections of 64 day-old control (Figure5A) and doxorubicin-treated (Figure5B-C) rats submitted to transferrin labeling. In the Figure 5A (control rat), intense transferrin labeling is observed in the Sertoli cell cytoplasm (thin arrows) in both seminiferous tubule sections (stages II-III and XIV). Sertoli cell nuclei (thick arrows) and elongated spermatids (arrowhead) are not labeled. The Figure 5B (64 day-old doxorubicin-treated rat) shows very weak transferrin labeling in the Sertoli cell cytoplasm of tubules at stages II-III and VII (thick arrow); however, no labeling is observed in the Sertoli cell nucleus (thin arrow). The Figure 5C depicts a seminiferous tubule cross-section showing intense germ cell depletion in which no transferrin labeling is observed. Interstitial tissue immunolabeling is also noted (Figures 5A; asterisks).