Figure 4

Testicular cross-sections of 40 day-old control (Figure4A) and doxorubicin-treated (Figure4B-C) rats submitted to transferrin labeling. The Figure 4A shows weak transferrin labeling in the Sertoli cell cytoplasm (arrows) of the control rat and strongly transferrin-positive germ cells, probably spermatogonia, which are located in the basis of the seminiferous epithelium (arrowheads). In the Figure 4B (doxorubicin-treated rat), although Sertoli cell cytoplasm is positive (arrows), no labeling is observed in the spermatogonia (arrowheads). The Figure 4C depicts a seminiferous tubule cross-section showing germ cell depletion and no transferrin labeling. Sertoli cell nucleus (arrow). Note the intense labeling in the interstitial tissue (Figures 4A-C; asterisks).