Testicular cross-sections of 127 day-old control (Figure3A) and doxorubicin-treated (Figures3B-3D) rats submitted to the PAS + H method. The Figure 3A depicts seminiferous tubule sections showing normal morphology. The Sertoli cells show normal nuclei (inset; arrowheads). In the Figure 3B, tubular sections with severe germ cell depletion and intraepithelial vacuoles (thin arrows) are seen. A displaced Sertoli cell nucleus from the basal membrane is also observed (thick arrow). Some Sertoli cell nuclei show abnormal profile (arrowheads). The Figure 3C shows Sertoli cell nuclei with abnormal morphology, showing round shape (thick arrows); in one of them the nucleolus is not evident. A binucleated formation of round spermatids can also be noted into the lumen (thin arrow). The Figure 3D depicts a portion of a seminiferous tubule showing a clear area without germ cells (arrows) and many primary spermatocytes in the tubular lumen (asterisk).