Table 1 Primer sets used in conventional and real-time PCR
Gene name (abbreviation) | GenBank accession no. | Primer sequence (5’ → 3’) | Product length (bp) |
|---|---|---|---|
Progesterone receptor (PR) | AY656812 | Forward: GATGCTATATTTTGCGCCTGA | 266 |
Reverse: CTCCTTTTTGCCTCAAACCA | |||
Progesterone receptor membrane component 1 (PGRMC1) | BC1184441 | Forward: GCAAGCTTTGGCGAAAATCA | 121 |
Reverse: CCCCTCGCATGTCCAATCAT | |||
Progesterone receptor membrane component 2 (PGRMC2) | NM_001099060 | Forward: AGGGGAAGAACCGTCAGAAT | 280 |
Reverse: AAGCCCCACCAGACATTACA | |||
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | NM_001034034 | Forward: GCTGAGGCTCCCATGTTTGT | 151 |
Reverse: TCATAAGTCCCTCCACGATGC | |||
Solute carrier family 30 (zinc transporter), member 6 (SLC30A6) | NM_001075766 | Forward: TGATGAGGAAACCTAGCCCTGCC | 143 |
Reverse: TCGGGCTGCTCCAAAAAGCGT | |||
Beta actin (ACTB) | NM_173979 | Forward: CTGGACTTCGAGCAGGAGAT | 140 |
Reverse: AGGAAGGAAGGCTGGAAGAG |