Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Table 2 Effects of cryoprotectant exposure, vitrification and time in the warming solution on cleavage of bovine oocytes and subsequent embryo development (Experiment 2)

Group	GV oocytes (n)	Cleavage rate n (%)	Blastocyst rate n (% of total)
Control (non-exposed)	81	61 (75.3%)^a	22 (27.2%)^a
VS1
1 min warming interval	82	60 (73.2%)^a	16 (19.5%)^a
5 min warming interval	77	48 (62.3%)^a	15 (19.5%)^a
VS1 + VS2
1 min warming interval	87	52 (59.8%)^a	16 (18.4%)^a
5 min warming interval	82	54 (65.9%)^a	22 (26.8%)^a
Vitrified
1 min warming interval	74	22 (29.7%)^b	1 (1.4%)^b
5 min warming interval	98	21 (21.4%)^b	2 (2.0%)^b
P-value
Cryoprotectant exposure		0.202	0.205
Warming time		0.241	0.689
Cryoprotectant exposure * warming time		0.381	0.687

Values with different superscripts (a,b) in the same column differ (P < 0.05).
VS1: Vitrification solution 1.
VS2: Vitrification solution 2.

ISSN: 1477-7827