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Table 2 Effects of cryoprotectant exposure, vitrification and time in the warming solution on cleavage of bovine oocytes and subsequent embryo development (Experiment 2)

From: Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Group GV oocytes (n) Cleavage rate n (%) Blastocyst rate n (% of total)
Control (non-exposed) 81 61 (75.3%)a 22 (27.2%)a
VS1    
 1 min warming interval 82 60 (73.2%)a 16 (19.5%)a
 5 min warming interval 77 48 (62.3%)a 15 (19.5%)a
VS1 + VS2    
 1 min warming interval 87 52 (59.8%)a 16 (18.4%)a
 5 min warming interval 82 54 (65.9%)a 22 (26.8%)a
Vitrified    
 1 min warming interval 74 22 (29.7%)b 1 (1.4%)b
 5 min warming interval 98 21 (21.4%)b 2 (2.0%)b
P-value    
Cryoprotectant exposure   0.202 0.205
Warming time   0.241 0.689
Cryoprotectant exposure * warming time   0.381 0.687
  1. Values with different superscripts (a,b) in the same column differ (P < 0.05).
  2. VS1: Vitrification solution 1.
  3. VS2: Vitrification solution 2.