Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Table 1 Effects of cryoprotectant exposure and vitrification on cleavage of bovine oocytes and subsequent embryo development (Experiment 1)

Group	GV oocytes (n)	Cleavage rate n (%)	Blastocyst rate n (% of total)
Control (non-exposed)	90	83 (92.2%)^a	31 (34.4%)^a
VS1	107	85 (79.4%)^a	27 (25.2%)^a
VS1 + VS2	96	77 (80.2%)^a	20 (20.8%)^a
Vitrified	127	52 (40.9%)^b	2 (1.6%)^b
P-value
Cryoprotectant exposure		0.847	0.456

Values with different superscripts (a,b) in the same column differ (P < 0.05).
VS1: Vitrification solution 1.
VS2: Vitrification solution 2.

ISSN: 1477-7827