Figure 2From: Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicusSDS-PAGE, Western blotting and native-PAGE analysis of purified cmFSH and cmLH. (A) 14% SDS-PAGE. Proteins reduced with 2-mercaptethanol and stained with Coomassie brilliant blue. (B) Western blotting with 003 antisera. (C) Western blotting with 299 antisera. (D) 7.5% native-PAGE. Proteins stained with Coomassie brilliant blue. Lane 1: A sample from the pooled fractions 13–19 in Figure 2 for cmFSH; Lane 2: A sample from the pooled fractions 20–26 in Figure 2 for cmLH.Back to article page