Skip to main content

Table 2 Treatment parameters for Study 1

From: Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system

Group name Sham Wet heat 1 MHz
high power
3 MHz
high power
3 MHz
high power
Na+
1 MHz
low power
1 MHz
low power
Na+
Group number 1 2 3 4 5 6 7
Coupling medium (°C) NR 45 37 37 37 NR NR
Treatments 2 2 2 2 2 2 2
Duration (minutes) 15 15 15 15 15 15 15
Coupling medium dg-DW dg-DW dg-DW dg-DW dg-Na+ dg-DW dg-Na+
Intensity (W/cm2) - - 2.2 2.2 2.2 1 1
Frequency (MHz) - - 1 3 3 1 1
Transducer (cm2) n/a n/a 5 5 5 10 10
Rotation n/a n/a + + + - -
Animals 2 3 3 4 4 3 3
  1. Degassed, 3% sodium chloride was used as the coupling medium (dg-Na+), otherwise degassed distilled water was used (dg-DW). Temperature of the coupling medium was noted; otherwise there was no regulation (NR). Transducer ME7413 had a surface area of ~ 5 cm2 (5) while model ME7410 had a surface area of ~ 10 cm2 (10). Transducers were stationary (-) or were rotated in a plane parallel to the bottom of the ultrasound chamber (+). All groups received two consecutive treatments separated by two days.