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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and placenta during pregnancy

Figure 6

Western blot analyses of endometrial and placentomal TRPV6 protein during pregnancy. A) Representative Western blot of TRPV6 protein expression in the uterine wall (n = 3 animals/month). Bands of approximately 75 kDa were detected. An additional band of approximately 90 kDa was seen in the 2nd and 3rd months. After incubation with N-glycosidase F (PNGase F), only the smaller band of 75 kDa was seen, demonstrating that the other band is the glycosylated variant of TRPV6. As a negative control, samples were preincubated with a control antigen. B) Relative expression of TRPV6 to GAPDH was determined by densitometry using ImageJ software. No significant changes were detected (p > 0.05). C) Representative Western blot analyses of TRPV6 in the placenta from 2nd to 9th months of pregnancy (n = 3 animals/month). D) The expression of TRPV6 relative to GAPDH was determined by measuring the optical density using ImageJ software. No significant changes (p > 0.05) were detected. Data were analysed using a parametric one-way analysis of variance (ANOVA), followed by Dunnett’s comparison test, where the second month was taken as the control group, and are presented as means ± SD.

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