Figure 4

Changes in peptide levels of GnRH1 (A), GnRH2 (B), and GnRH3 (C) in the brain of adult chub mackerel during different stages of spawning cycle. Each bar represents mean ± SEM from 4–6 fish per ovarian stage (Refer Table 1). Different letters above the bars represent significant differences (p<0.05) between stages. LV, late vitellogenesis; GVM, germinal vesicle migration; HY, hydration; OV, ovulation; POV, post-ovulation.