In vitro and in vivodevelopment of mice morulae after storage in non-frozen conditions

Table 2 Total cell count of blastocysts developed after morulae ( in vivo developed) storage during 48 hours

Treatment		Number of blastocysts analysed	Total cell (N° of cells)
Media	Temperature	Number of blastocysts analysed	Total cell (N° of cells)
Control	37°C	23	69.43 ± 3.19 ^a
KSOM	4°C	15	64.67 ± 1.97 ^a
KSOM-HEPES	4°C	21	70.05 ± 2.80 ^a
KSOM	15°C	14	41.14 ± 2.66 ^b
KSOM-HEPES	15°C	23	43.30 ± 2.12 ^b
KSOM	37°C	39	52.97 ± 1.39 ^c
KSOM-HEPES	37°C	22	38.15 ± 1.47 ^b

Mean ± SEM. Different letters indicate significant differences between treatments. ANOVA followed by Student-Newman-Kleus post hoc test was performed.

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