Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the Barred Frog Mixophyes fasciolatus (Myobatrachidae)

Table 4 Effect of diurnal cycle on timing of oviposition by M. fasciolatus

	Treatment Group/Number of ovipositions observed
Oviposition time (diurnal cycle)	hCG only	hCG and pituitary glands	PMSG and hCG	N
0 – 12 hours*	9	2	30	41
12 – 24 hours**	1	0	2	3

Effect of diurnal cycle on timing of oviposition by M. fasciolatus. Data from Table 3. No significant effect of treatment group on timing of oviposition (p = 0.625); significant effect of stage of diurnal cycle on oviposition timing (p<0.0001). * 0–12 hours = 0:00–6:00 hr dark, 6:00–12:00 hr light; **12-24 hours = 12:00–18:00 hr light, 18:00–24:00 hr dark.

ISSN: 1477-7827