Figure 8

The expression of Src, p-ERK1/2, p-PKC and p-PI3K protein after c-src siRNA, PD98059, Calphostin and LY294002 treatment, respectively. A,The expression of Src, p-ERK1/2, p-PKC and p-PI3K protein in the ovaries detected by western blotting. B, The expression of Src protein according to a semiquantitative analysis of the western blot result. C, The expression p-ERK1/2 protein according to a semiquantitative analysis of the western blot result. D, The expression of p-PKC protein according to a semiquantitative analysis of the western blot result. E, The expressions of p-PI3K protein by semiquantitative analysis of the western blot result. Blank control corresponds to the group transfected with negative control siRNA. The data are presented as the means ± SEM (n = 3). ** P<0.01 vs. blank control group.