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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways

Figure 2

The silencing efficiency of the lentivirus after transfection. A, c-src mRNA in ovaries 8 days after lentivirus transfection and a semiquantitative assay of the c-src mRNA levels 8 days after lentiviral transfection from six different replicates. B, Real-time PCR was used to analyze the relative c-src mRNA (c-src mRNA/β-actin mRNA). Blank control corresponds to the group that was transfected with c-src-non-targeting oligonucleotides, negative control corresponds to the group that was transfected with a blank vector (lentivirus without siRNA), and c-src siRNA corresponds to the group with the lentivirus packaging c-src siRNA. The data are presented as the means ± SEM (n = 3). **P<0.01 compared with the negative control group.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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