Figure 4

A representative picture showing total numbers of cells examined under fluorescence (FITC, 512 nm) for AOP-1: A-C) Day 7 bovine blastocysts derived from oocytes exposed to BoHV-5 infection showing marked signals to AOP-1; D-E) Day 7 blastocysts derived from oocytes unexposed to BoHV-5 infection showing mild signals to AOP-1. Electrophoresis agarose gel of AOP-1 RT-PCR: MW-molecular weight 1-kb plus; lane 1 represents RT-PCR results from pure water used as a negative control; lane 2 shows the AOP-1 negative RT-PCR amplification of unexposed embryos (310 bp); lane 3 shows the positive RT-PCR amplification of exposed embryos (310 bp). Scale bar is equal to 100 μm.