Figure 1

Day 7 in vitro cultured bovine blastocysts derived from control oocytes (unexposed to BoHV-5) and exposed oocytes. A) DAPI labeling (425 nm) under fluorescence shows the good quality of the exposed embryos; B) Indirect immunofluorescence assay showing positive signals to BoHV-5 antigens under fluorescence (512 nm). Scale bars is equal to100 μm; C) An electrophoresis agarose gel of US9 PCR: MW-molecular weight 1-kb plus; lanes 1 and 2 represent PCR results from unexposed oocytes and bovine semen; lane 3 shows the positive PCR amplification of 7-day embryos derived from exposed oocytes (250 bp); lane 4 shows the negative PCR amplification of embryos derived from unexposed oocytes.