Table 1 Oligonucleotide sequences used in RT-PCR analysis
From: Developmental expression and function of DKKL1/Dkkl1 in humans and mice
Transcripts | Annealing Temperature (°C) | Product size (bp) | Sequence direction (5’-3’) |
|---|---|---|---|
DKKL1 | 58 | 299 | Sense: TGCTGCTCCTCTCTACCCT |
Antisense: CTCTCCTGTCTTGTTGTCGG | |||
Dkkl1 | 55 | 217 | Sense: TCGTGTCCTCCTCTGCTCTCT |
Antisense: TTGCCCATTCTGTGCTCCT | |||
β-actin | 55 | 281 | Sense: AACAGTCCGCCTAGAAGCAC |
Antisense: CGTTGACATCCGTAAAGACC | |||
GAPDH | 58 | 100 | Sense: GCTCTCTGCTCCTCCTGTTC |
Antisense: GACTCCGACCTTCACCTTCC |