Figure 1

Developmental expression pattern of DKKL1/Dkkl1 during spermatogenesis detected by Affymetrix chip analysis. A: Mice testis was isolated from postnatal Balb/C mice on days 4, 9, 18, 35, and 54, as well as on 6 months and applied to whole genomic analysis by Affymetrix chip. The scaling signal intensities of Dkkl1 from mouse testis on days 4, 9, 18, 35, and 54, as well as on 6 months were 1.8, 9.9, 1030.5, 2696.8, 2987.9 and 2752.7, respectively. Signals on day 4 and 9 were not detected. On the other hand, the signal intensities of β-actin were 3688.8, 3764.78, 3812.9, 3696.87, 3679.71 and 3757.12, respectively. B: Hybridization using a human Affymetrix Genechip revealed differential expression of DKKL1 in human foetal (6 months) and adult (27 yr) testes. The hybridization intensity of DKKL1 in foetal and adult testes was 5 and 2,027.8, respectively, and the signal intensity of β-actin was 987.4 and 760.8, respectively.