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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: PreImplantation Factor (PIF) promoting role in embryo implantation: increases endometrial Integrin-α2β3, amphiregulin and epiregulin while reducing betacellulin expression via MAPK in decidua

Figure 1

sPIF promotes endometrial receptivity by up-regulating α2β3 integrin. Endometrial epithelial cells were isolated and cultured for 5 days in presence of FCS followed by 24hrs in serum-free media containing 1–125 nM sPIF (15, or 9 aa). At the end of culture cells stained with specific antibody and α2β3 integrin expression was then determined by flow cytometry. Nonspecific IgG isotype served as negative control. a) PIF15 promotes α2β3 integrin in a dose dependent manner as compared with scrambled PIF 125nM or 2%BSA, effect was not significant. b) PIF9 promotes endometrial-α2β3 integrin in a dose dependent manner as compared with 2%BSA, control. Data expressed as (Mean ± SEM.) percent change in basal antigen expression in epithelial cells as compared to negative controls. Data are representative of three different experiments with similar results (ANOVA, *P < 0.05).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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