Table 2 Primers and Probes for qPCR and ISH
From: Sex differentiation in Atlantic cod (Gadus morhua L.): morphological and gene expression studies
Transcript | Primer or probe | Sequence (5′–3′) |
|---|---|---|
amh | forward primer | GTCAGGCCAGCGAGAGCA |
reverse primer | AGGGCGACAACACATACGTTTC | |
probe | [FAM]-CATCTGCAGGTGCAGGAACACTATATGC-[TAMRA] | |
cyp19a1a | forward primer | ACAACAACAAGTACGGCAGCAT |
reverse primer | GTAGAGGAGCTGCTGAGGATGAG | |
probe | [FAM]-CGGCGTATGGATCAA-[MGB] | |
ef1á | forward primer | GCCCCTCCAGGACGTCTAC |
reverse primer | ACGGCCCACGGGTACTGT | |
probe | [FAM]-AGATCGGCGGTATTG-[MGB] | |
dax1 | forward primer | TGGTGGCGCAGCTCTTCT |
reverse primer | GCAGCACCTCTTCCATGTTGA | |
probe | [FAM]-CAAGCCCGTGATCGGCGCC-[TAMRA] | |
shp | forward primer | GCGGGCGTCGGTAAACTTA |
reverse primer | CGCGTACTCCTTCGGACTCA | |
probe | [FAM]- CAAACCGCCAAACCTCTTGAGACAGGGCCAAAC -[TAMRA] | |
sox9b | forward primer | AAGAAGCCGAGCGCTTAAGG |
reverse primer | CCGCCTCGGTTGGTATTTG | |
probe | [FAM]-TTCAACACAAAAAGGACCACCCGGACT-[TAMRA] | |
vasa | forward primer | T3Rpps- TGACCTGGACCAGCTTCTGCTCCACGTCA |
reverse primer | T7Rpps- CAAGTTTGCTCATGGGACCTGCGTGCGT |