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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Reduced osteoblast activity in the mice lacking TR4 nuclear receptor leads to osteoporosis

Figure 2

TR4−/−bone marrow cultures have reduced osteoblast differentiation. Bone marrow cells were isolated from 10 weeks old TR4−/− and control TR4+/+ mice. Cells were cultured in 2 mL α-MEM containing 10% FBS at 5x106 cells/well in 6-well plates. After 7 days, the media was replaced with media containing β-glycerophosphate and ascorbic acid to induce osteoblast differentiation. Colonies were stained on day 21 after plating by alkaline phosphatase (A) and alizarin red ( B). The assays were performed in triplicate.

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