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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Phosphorylation of h1 Calponin by PKC epsilon may contribute to facilitate the contraction of uterine myometrium in mice during pregnancy and labor

Figure 2

The expression of PKC-ε and phosphorylated PKC-ε during different stages of pregnancy in mice. Total protein extracts from the smooth muscle layer of mouse uterus was analyzed on SDS-PAGE and transferred to nitrocellulose membrane for western blotting with the anti-PKC-ε and anti-phosphorylated PKC-ε antibody. A: blots show the expression of PKC-ε and phosphorylated PKC-ε in the myometrium in different stages of pregnancy. B: columns from densitometry quantification of multiple western blots (means ± SD; n = 10) summarize the changes in the expression of PKC-ε and phosphorylated PKC-ε. C: curves from the ratio of phosphorylated PKC-ε to total PKC-ε. NP: non-pregnant; D7: Day 7 in pregnancy; D12: Day 12 in pregnancy; D17: Day 17 in pregnancy; D18: Day 18 in pregnancy; D19: Day 19 in pregnancy; IL: in labor. *: P < 0.05, compared with NP; **: P < 0.01, compared with NP; Δ: P < 0.05, compared with NP; ΔΔ: P < 0.01, compared with NP. Results demonstrated that total PKC-ε expression level gradually increased as the pregnancy progression, reached the peak before labor and continued until labor; the phosphorylation of PKC-ε expression levels increased gradually with the progress of pregnancy, reached the peak after labor. The ratio of phosphorylated PKC-ε to the total PKC-ε had no significant changes during pregnancy, but reached a peak after the onset of labor.

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