Three dimensional culture of fresh and vitrified mouse pre-antral follicles in a hyaluronan-based hydrogel: a preliminary investigation of a novel biomaterial for in vitro follicle maturation

Table 1 HA gel concentration and follicle maturation

	2 mg/ml	3 mg/ml	4 mg/ml	5 mg/ml	P-value
Follicles initially plated	60	60	60	60
Follicles retained in gel	34	48	36	44
Survival * (%)	22 (65%)	24 (50%)	16 (44%)	18 (41%)	NS **
GVBD (%)	20 (91%)	24 (100%)	11 (69%)	15 (83%)	P < 0.05
MII (%)	12 (55%)	14 (58%)	7 (44%)	8 (44%)	NS

*Follicles with intact oocytes surviving 12 days in culture. Remaining follicles failed to develop and underwent atresia.
**Linear trend towards better survival with lower HA concentrations (P = 0.03). Follicles embedding in drop format.

ISSN: 1477-7827