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Table 1 HA gel concentration and follicle maturation

From: Three dimensional culture of fresh and vitrified mouse pre-antral follicles in a hyaluronan-based hydrogel: a preliminary investigation of a novel biomaterial for in vitro follicle maturation

  2 mg/ml 3 mg/ml 4 mg/ml 5 mg/ml P-value
Follicles initially plated 60 60 60 60  
Follicles retained in gel 34 48 36 44  
Survival * (%) 22 (65%) 24 (50%) 16 (44%) 18 (41%) NS **
GVBD (%) 20 (91%) 24 (100%) 11 (69%) 15 (83%) P < 0.05
MII (%) 12 (55%) 14 (58%) 7 (44%) 8 (44%) NS
  1. *Follicles with intact oocytes surviving 12 days in culture. Remaining follicles failed to develop and underwent atresia.
  2. **Linear trend towards better survival with lower HA concentrations (P = 0.03). Follicles embedding in drop format.