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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Three dimensional culture of fresh and vitrified mouse pre-antral follicles in a hyaluronan-based hydrogel: a preliminary investigation of a novel biomaterial for in vitro follicle maturation

Figure 3

Morphology of two layered preantral follicles embedded in 3 mg/ml HA gel or HA combined with extracellular matrix (ECM-HA) is depicted. (A,G) Follicles from both culture models 24 hours after embedding. Follicle morphology on Day 7 in HA gel alone (B-D) or in ECM-HA (H-J). HA- embedded follicles grew in a more spherical fashion, with an intact basement membrane and thecal cell (TH) layer. Antral cavity (AC) formation is clearly visible (arrow). Follicle morphology in ECM-HA was quite different from HA alone but a 3-D growth pattern was maintained. By Day 12 of culture (E-F and K-L), follicles in both HA and ECM-HA gels formed antrums (arrows). Magnification 40X (Day 1), 200X (Days 7 and 12).

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