Figure 2

Photomicrographs of seminal vesicle of control and kisspeptin treated prepubertal rats. a-b: Control seminal vesicle showing complex glandular structure. The lumen (L) is highly irregular having crypts (C). The epithelial cells (EC) are columnar or pseudostratified columnar resting on lamina muscularis (LM). c-d: Seminal vesicle of rats treated with 10 pg kisspeptin. The structure still represents complex foldings, is irregular with dilated lumen as compared to the control. The epithelium is still columnar. e-f: Seminal vesicle of rats treated with 1 ng kisspeptin. Seminal vesicles show loss of intricate foldings (arrow) while the lumen is well dilated. The glandular epithelium is not well developed and height of columnar cells appears greatly reduced. g-h: At 1 μg kisspeptin dose the lumen has significantly dilated, while the branching of the mucosa of seminal vesicles was distinctly reduced. Compared to low doses of kisspeptin, the epithelial cells are no longer columnar. They were either cuboidal or squamous shape. Scale bar left panel = 50 μm, right panel = 20 μm.