Figure 3

Effect of resveratrol on cell-death machinery in cultured rat GCs. Effect of resveratrol on mRNA levels of (A) Bcl-2 and (B) Bax was investigated by quantitative real-time RT-PCR. The mRNA level of the untreated control was arbitrarily set at 1.0, and that of the treatment group was estimated relative to the control value. Results are shown as the mean ± SEM (bars) of three independent experiments. (C) Caspase-3/7 activity was measured by the Apo-ONE Homogeneous Caspase-3/7 Assay kit at 6, 12 and 24 h. Results are shown as the mean percentage of the untreated control ± SEM (bars) of eight wells of three independent experiments. (D) Hoechst 33342 staining of resveratrol-treated rat GCs at 6, 12, 24, and 48 h. * p < 0.05 vs. control. ** p < 0.01 vs. control.